This Guide contains detailed information on installing, configuring and using cryoSPARC v0.2.47 to 0.6.5. For detailed documentation, installation instructions and a tutorial for cryoSPARC v2.0+, please visit our new documentation page at We highly recommend upgrading to v2.0 to take advantage of the full pipeline for processing single particle cryo-EM data, as well as powerful new workflow features.

Configure users

Add new user

Each user in the lab should have their own cryoSPARC account so that datasets, results, etc, can be viewed separately. To add a new user, log out of cryoSPARC by clicking on the user profile. Then, click “Sign Up” and enter the new user’s information.


Access cryoSPARC Settings by clicking on the user name on the header. Users can set experiments to open in a new tab and enable notifications.