Downloads: Workflow JSONs, sample volumes, and masks

Dedicated space for all downloads related to automated workflows for CryoSPARC

Workflow v1

This Workflow v1 was tested using 21 GPCR datasets. The development of the Workflow is described here:

Full automated data processing: a case study using GPCRs

Read the entire preprint here: End-to-end automation of repeat-target cryo-EM structure determination in CryoSPARC

Below we provide a link to download a .zip archive containing CryoSPARC Workflow JSON files and required inputs which can be imported into CryoSPARC v4.7.1+ and used to process GPCR datasets like the ones we have tested. Two versions of the Workflow file are provided: one that processes raw movies directly from import, and another that processes micrographs that are generated after pre-processing in CryoSPARC Live. Please refer to the preprint for details.

Download Workflow v1 files: automated_workflow_materials_v1.zip

Workflow v2

We've continued refining and simplifying the above workflow, and now we've put it to the test on nine datasets of CDK-activating Kinase (CAK) where we overcome severe preferred orientation resulting in maps with little to no anisotropy. Below you will find the workflow JSON file, the reference volume, and the junk volumes used in this case study. Additionally, the resulting maps and models from the automated data processing of CAK are available for download.

Download Workflow v2 files: automated_workflow_materials_v2.zip

Download CAK automation results: CAK_automation_maps_models.zip