Job: MotionCor2 (Wrapper) (BETA)
How to use the wrapper for MotionCor2 available in CryoSPARC.
Description
An algorithm to correct anisotropic image motion at the single pixel level across the whole frame, suitable for both single particle and tomographic images. Iterative, patch-based motion detection is combined with spatial and temporal constraints and dose weighting. Please ensure you review the MotionCor2 License Terms below.
Input
Raw movies (with microscope parameters & gain reference files imported earlier)
Common Parameters
Path to MotionCor2 executable:
The absolute path to the MotionCor2 binary. Note: Runcryosparcm cli 'set_project_param_default(proect_uid, 'exec_param', path)'
(where project_uid is your project number (e.g 'P12') and path is the absolute path to the MotionCor2 binary) to keep this parameter consistent across this project.
Output
Global and local (patch-based) motion corrected dose weighted micrographs
Global and local (patch-based) motion corrected non-dose weighted micrographs (for CTF Estimation)
Global and local (patch-based) motion trajectories
MotionCor2 License Terms
Common Next Steps
CTF Estimation (ensure non-dose weighted micrographs are used)
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