This Guide contains detailed information on installing, configuring and using cryoSPARC v0.2.47 to 0.6.5. For detailed documentation, installation instructions and a tutorial for cryoSPARC v2.0+, please visit our new documentation page at We highly recommend upgrading to v2.0 to take advantage of the full pipeline for processing single particle cryo-EM data, as well as powerful new workflow features.


Cryo-EM Single Parcticle Ab initio Reconstruction and Classification (cryoSPARC)

CryoSPARC is a software package for processing single-particle electron cryo-microscopy (cryo-EM) data.

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