How to Download, Install and Configure
Meeting system requirements, obtaining a License ID, and downloading & installing cryoSPARC.

Step 1: Confirm Prerequisites

Ensure you have reviewed and completed the pre-requisites before downloading and installing cryoSPARC:
Once you have confirmed the prerequisites, you should have completed the following:
  • Create a shared user
  • Configure SSH access
  • Ensure all required ports are open
  • Set up a shared file system
  • Install CUDA (requires version ≥10)
In addition, make sure that the hardware to which you are installing meets system requirements:

Step 2: Obtain a cryoSPARC License ID

CryoSPARC is available free of charge for non-profit academic use. To download the software, you will need a cryoSPARC License ID, which can be requested via the form at
Please contact [email protected] for inquiries relating to for-profit or industry licensing, including academic-industry collaborations on proprietary projects and fee-for-service data processing.

Step 3: Download and Install cryoSPARC


Once you've reviewed the cryoSPARC Installation Prerequisites and have decided on an architecture suitable for your situation, the installation process can be distilled to a few main steps:
  1. 1.
    Download the cryosparc_master package
  2. 2.
    Download the cryosparc_worker package
  3. 3.
    Install the cryosparc_master package on the master node
  4. 4.
    Start cryoSPARC for the first time
  5. 5.
    Create the first administrator user
  6. 6.
    [Optional] Set up recurring backup of the cryoSPARC database
  7. 7.
    If you chose to install cryoSPARC via the single-workstation method, at this point, you are finished installing cryoSPARC. Otherwise, continue.
  8. 8.
    Log onto a worker node and install the cryosparc_worker package (installing the cryosparc_worker package requires an NVIDIA GPU and CUDA Toolkit 10+)
  9. 9.
    Depending on whether you're installing cryoSPARC on a cluster or on a standalone worker machine, connect the worker node to cryoSPARC via cryosparcm cluster connect or cryosparcw connect
  10. 10.
    CryoSPARC is now fully installed. You may also choose to add more standalone worker machines at any time by using the cryosparcw connect utility.
You will need at least 15 GB of space to download and install the cryosparc_master and cryosparc_workerpackages

Detailed Installation Steps