Job: Full-Frame Motion Correction

Full-frame motion correction.

Description

Full-frame motion correction estimates and corrects for full-frame motion (e.g., stage drift) in movie data. Two versions are available - the first runs on a single GPU, while the multi option uses multiple available GPUs to parallelize motion correction.

Input

• Raw movies in .mrc or .tiff format

Common Parameters

• Only process this many movies: Selects the first n movies to process, instead of processing the entire set. Helpful when working with a set of movies for the first time, to better understand the data quality.

• Low GPU memory: When working with very large movies on GPUs with low memory (less than, say, 16 GB), it is possible to run out of GPU memory. If this happens, turning on low-memory mode will usually allow the processing to proceed. This option makes processing slightly slower, so it is turned off by default.

• Save results in 16-bit floating point: Saves the output micrographs in float16 format (CryoSPARC v4.4+).

Output

• Aligned/motion-corrected micrographs

• Rigid motion trajectory estimates

Common Next Steps

• CTF Estimation