Last updated
Last updated
Heterogeneous Refinement simultaneously classifies particles and refines structures from n initial structures, usually obtained following an Ab-Initio Reconstruction. This facilitates the ability to look for small differences between structures which may not be obvious at low resolutions, and also to re-classify particles to aid in sorting.
Initial models
Particles
Refinement box size (voxels):
Symmetry:
You may wish to enforce a particular type of symmetry during refinement. Common types of symmetry include T (tetrahedral), O (octahedral), I (icosahedral), D (dihedral) and C (cyclic).
Use spherical mask
(new in v4.5): Default is on with a corner window mask. User can control the inner and outer diameter of the mask with parameters Spherical mask diameter (A)
and Spherical mask diameter outer (A)
. If only the inner diameter is set, the outer diameter is default to be 20 percent larger than the inner diameter. The window mask transitions smoothly between inner and outer diameters.
All particles
All volumes
Note that this is a output, new in CryoSPARC v4.5. It includes a series
result containing a downloadable zip file of all volumes.
Particles for each class
Volume for each class
Including Half-maps
Plots, including orientation distribution for each class
This task enables simultaneously sorting particles and identifying classes and is particularly useful in cases where identified classes look very similar. This task can also be used as a method to remove “junk” particles.
The initial models provided must be on the correct grey scale. Outputs from ab-initio reconstruction in CryoSPARC meet this requirement.
Download and inspect map
Heterogeneous refinement.