Job: Heterogeneous Refinement
Heterogeneous refinement.
Description
Heterogeneous Refinement simultaneously classifies particles and refines structures from n initial structures, usually obtained following an Ab-Initio Reconstruction. This facilitates the ability to look for small differences between structures which may not be obvious at low resolutions, and also to re-classify particles to aid in sorting.
Input
Initial models
Particles
Common Parameters
Refinement box size (voxels):
Symmetry:
You may wish to enforce a particular type of symmetry during refinement. Common types of symmetry include T (tetrahedral), O (octahedral), I (icosahedral), D (dihedral) and C (cyclic).
Output
Refined 3D maps
Half-maps
Mask used in refinement
Mask used in FSC calculation
Gold-standard FSC curve
Plots, including orientation distribution
Notes
This task enables simultaneously sorting particles and identifying classes and is particularly useful in cases where identified classes look very similar. This task can also be used as a method to remove “junk” particles.
Limitations
The initial models provided must be on the correct grey scale. Outputs from ab-initio reconstruction in CryoSPARC meet this requirement.
Common Next Steps
Download and inspect map
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