Job: Create Templates

Create templates for particle picking.

Description

Generate projection-based templates from a given input volume. Templates are generated from evenly sampled projection directions around the encompassing sphere, as according to the SK97 sampling algorithm.

Input

• Volume

Common Parameters

• Number of equally-spaced templates to generate

• Zeropadding factor for interpolation

  • This is the multiplicative factor that determines the internal box size, by multiplying the original box size; the real-space volume is zeropadded out to this size

  • Higher zeropadding factors can increase the interpolation quality, at the expense of increased CPU memory

• Output size

  • The desired box size of the output templates

Output

• Templates (blob)

Examples

Below is an example of generating 20 equally spaced templates from a refined T20S Proteasome structure.

The templates generated from projections of a T20S proteasome

Common Next Steps

The most common use of templates is for subsequent particle picking from micrographs, using the Template Picker job. You may also use a Select 2D job to subset certain templates from the output stack.