Job: Create Templates
Create templates for particle picking.
Generate projection-based templates from a given input volume. Templates are generated from evenly sampled projection directions around the encompassing sphere, as according to the SK97 sampling algorithm.
Number of equally-spaced templates to generate
Zeropadding factor for interpolation
- This is the multiplicative factor that determines the internal box size, by multiplying the original box size; the real-space volume is zeropadded out to this size
- Higher zeropadding factors can increase the interpolation quality, at the expense of increased CPU memory
- The desired box size of the output templates
- Templates (
Below is an example of generating 20 equally spaced templates from a refined T20S Proteasome structure.
The templates generated from projections of a T20S proteasome