Job: Create Templates
Create templates for particle picking.
Generate projection-based templates from a given input volume. Templates are generated from evenly sampled projection directions around the encompassing sphere, as according to the SK97 sampling algorithm.
- Volume
Number of equally-spaced templates to generateZeropadding factor for interpolation- This is the multiplicative factor that determines the internal box size, by multiplying the original box size; the real-space volume is zeropadded out to this size
- Higher zeropadding factors can increase the interpolation quality, at the expense of increased CPU memory
Output size- The desired box size of the output templates
- Templates (
blob)
Below is an example of generating 20 equally spaced templates from a refined T20S Proteasome structure.
The templates generated from projections of a T20S proteasome
The most common use of templates is for subsequent particle picking from micrographs, using the Template Picker job. You may also use a Select 2D job to subset certain templates from the output stack.
Last modified 1yr ago