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CryoSPARC Guide
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CryoSPARC Guide
About cryoSPARC
Current Version
Licensing
Setup, Configuration and Management
Hardware and System Requirements
cryoSPARC Installation Prerequisites
How to Download, Install and Configure
Software Updates
Management and Monitoring
Troubleshooting
Deployment Guides
Deploying cryoSPARC on AWS
Processing Data in cryoSPARC
Cryo-EM Data Processing in cryoSPARC: Introductory Tutorial
User Interface and Usage Guide
All Job Types in cryoSPARC
Tutorial Videos
Tutorials and Case Studies
Tutorial: Verify cryoSPARC Installation with the Extensive Workflow (Sysadmin Guide)
Tutorial: User Management
Tutorial: Job Builder
Case Study: Processing EMPIAR-10291 (300 Micrographs) to 3.4Ã… in 1 hour 25 minutes
Tutorial: Negative Stain Data
Tutorial: Phase Plate Data
Tutorial: EER File Support
Tutorial: Patch Motion and Patch CTF
Tutorial: Manually Curate Exposures
Tutorial: Particle Picking Calibration
Tutorial: CTF Refinement
Case Study: Yeast U4/U6.U5 tri-snRNP
Tutorial: 3D Variability Analysis (Part One)
Tutorial: 3D Variability Analysis (Part Two)
Tutorial: Queuing Directly to a GPU
Tutorial: SSD Particle Caching in cryoSPARC
Tutorial: Priority Job Queuing
Tutorial: Data Management in cryoSPARC
Tutorial: CryoSPARC Live Session Data Management
Tutorial: Manipulating .cs Files Created By cryoSPARC
Tutorial: Migrating your cryoSPARC Instance
Performance Metrics
Real-time processing in cryoSPARC Live
About cryoSPARC Live
Prerequisites and Compute Resources Setup
How to Access cryoSPARC Live
UI Overview
Live Jobs and Session-Level Functions
New Live Session: Start to Finish Guide
Tutorial Videos
Performance Metrics
FAQs and Troubleshooting
Cryo-EM Data Processing Explained
Questions and Support
cryoSPARC Discussion Forum
cryoSPARC Blog
cryoSPARC Homepage
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Tutorials and Case Studies

Installation, Setup and User Management

Introduction to cryoSPARC

Data Processing

Alongside these tutorials, see: All Job Types in cryoSPARC and Tutorial Videos​

Files, Data and Import

Preprocessing

Particle Picking

Ab-Initio Reconstruction

Case Study: Previously unknown conformation of AAA+ unfoldase discovered using cryoSPARC

https://cryosparc.com/blog/aaa-discovered-using-cryosparc
cryosparc.com

Helical Reconstruction

3D Refinement

Local Refinement

Variability Analysis

Software and Data Management

Software Utility Features

Data Management, Import and Export

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Tutorial: Verify cryoSPARC Installation with the Extensive Workflow (Sysadmin Guide)
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Contents
Installation, Setup and User Management
Introduction to cryoSPARC
Data Processing
Files, Data and Import
Preprocessing
Particle Picking
Ab-Initio Reconstruction
Helical Reconstruction
3D Refinement
Local Refinement
Variability Analysis
Software and Data Management
Software Utility Features
Data Management, Import and Export