Data Processing Tutorials
Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.
Last updated
Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.
Last updated
Processing EMPIAR-10853 including Micrograph Denoising, Micrograph Junk Detector, Subset Particles by Statistic, Local Refinement and focussed 3D Classification to improve ligand density.
Processing EMPIAR-10059 in CryoSPARC v4.6 with a focus on particle curation, developing an intuition for when a domain may be blurred due to flexibility, and how to handle this type of flexibility.
Processing EMPIAR-10256 with a focus on handling pseudosymmetry and 3D Classification parameter choices.
Processing EMPIAR-10668 including Local Refinement, 3D Variability Analysis, 3D Flex and classification of continuous heterogeneity.
Processing EMPIAR-10716 with a focus on high-symmetry ab-initio reconstruction, local symmetry, non-point-group symmetry, symmetry expansion, and custom geometry operations.
An analysis of the impact of data collection and particle picking techniques on orientation bias, including strategies for improving map quality and isotropy.
Processing EMPIAR-10073 with a focus on Local Refinement.
Friendly, approachable introductions to cryoEM data processing in CryoSPARC with a focus on the exploratory, "try-it-and-see" nature of single-particle analysis. Kindly reproduced from Oliver Clarke, PhD, Assistant Professor of Physiology and Cellular Biophysics at Columbia University.
Helpful hints for processing cryo-EM data of membrane proteins.
A detailed description of the common plots that CryoSPARC makes across multiple job types.