Data Processing Tutorials
Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.
Last updated
Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.
Last updated
Processing EMPIAR-10853 including Micrograph Denoising, Micrograph Junk Detector, Subset Particles by Statistic, Local Refinement and focussed 3D Classification to improve ligand density.
Processing EMPIAR-10059 in CryoSPARC v4.6 with a focus on particle curation, developing an intuition for when a domain may be blurred due to flexibility, and how to handle this type of flexibility.
Processing EMPIAR-10256 with a focus on handling pseudosymmetry and 3D Classification parameter choices.
Processing EMPIAR-10668 including Local Refinement, 3D Variability Analysis, 3D Flex and classification of continuous heterogeneity.
Processing EMPIAR-10716 with a focus on high-symmetry ab-initio reconstruction, local symmetry, non-point-group symmetry, symmetry expansion, and custom geometry operations.
Processing a combined dataset of an ion channel with a focus on techniques for separating conformational states from a heterogeneous mixture.
An analysis of the impact of data collection and particle picking techniques on orientation bias, including strategies for improving map quality and isotropy.
Processing EMPIAR-10073 with a focus on Local Refinement.
Friendly, approachable introductions to cryoEM data processing in CryoSPARC with a focus on the exploratory, "try-it-and-see" nature of single-particle analysis. Kindly reproduced from Oliver Clarke, PhD, Assistant Professor of Physiology and Cellular Biophysics at Columbia University.
Helpful hints for processing cryo-EM data of membrane proteins.
A detailed description of the common plots that CryoSPARC makes across multiple job types.