Data Processing Tutorials

Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.

Introduction to CryoSPARC
User Interface and Usage Guide
Get Started with CryoSPARC: Introductory Tutorial (≤v3.3)
Tutorial: Job Builder (≤v3.3)
Case Studies
Case Study: Processing EMPIAR-10291 (300 Micrographs) to 3.4Å in 1 hour 25 minutes
Tutorial: Tips for Membrane Protein Structures
Data Processing
Alongside these tutorials, see: All Job Types in CryoSPARC and Tutorial Videos​
Files, Data and Import
Tutorial: Negative Stain Data
Tutorial: Phase Plate Data
Tutorial: EER File Support
Preprocessing
Tutorial: Patch Motion and Patch CTF
Tutorial: Manually Curate Exposures (≤v3.3)
Particle Picking
Tutorial: Particle Picking Calibration
Tutorial: Blob Picker Tuner
Ab-initio Reconstruction
Case Study: Previously unknown conformation of AAA+ unfoldase discovered using cryoSPARC
https://cryosparc.com/blog/aaa-discovered-using-cryosparc
cryosparc.com
Helical Reconstruction
Tutorial: Helical Processing using EMPIAR-10031 (MAVS)
3D Refinement
Tutorial: Maximum Box Sizes for Refinement
Tutorial: CTF Refinement
Tutorial: Ewald Sphere Correction
Local Refinement
Tutorial: Mask Selection and Generation in UCSF Chimera
Case Study: Yeast U4/U6.U5 tri-snRNP
Conformational Variability
Tutorial: 3D Classification (BETA)
Tutorial: 3D Variability Analysis (Part One)
Tutorial: 3D Variability Analysis (Part Two)
Tutorial: 3D Flexible Refinement
​

Processing Data in cryoSPARC - Previous

CryoSPARC Tools

Tutorial: Job Builder (≤v3.3)

Last modified 3mo ago