Data Processing Tutorials
Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.
Introduction to CryoSPARC
pageUser Interface and Usage GuidepageGet Started with CryoSPARC: Introductory Tutorial (≤v3.3)pageTutorial: Job Builder (≤v3.3)Case Studies
pageCase Study: Exploratory data processing by Oliver ClarkepageCase Study: Processing EMPIAR-10291 (300 Micrographs) to 3.4Å in 1 hour 25 minutespageTutorial: Tips for Membrane Protein StructurespageTutorial: Common CryoSPARC PlotsData Processing
Alongside these tutorials, see: All Job Types in CryoSPARC and Tutorial Videos
Files, Data and Import
pageTutorial: Negative Stain DatapageTutorial: Phase Plate DatapageTutorial: EER File SupportpageTutorial: EPU AFIS Beam Shift ImportPreprocessing
pageTutorial: Patch Motion and Patch CTFpageTutorial: Manually Curate Exposures (≤v3.3)pageTutorial: Float16 SupportParticle Picking
pageTutorial: Particle Picking CalibrationpageTutorial: Blob Picker TunerAb-initio Reconstruction
Case Study: Previously unknown conformation of AAA+ unfoldase discovered using cryoSPARC
Helical Reconstruction
pageTutorial: Helical Processing using EMPIAR-10031 (MAVS)3D Refinement
pageTutorial: Maximum Box Sizes for RefinementpageTutorial: CTF RefinementpageTutorial: Ewald Sphere CorrectionpageTutorial: Symmetry RelaxationpageTutorial: Orientation DiagnosticspageTutorial: BILD files in CryoSPARC v4.4+Local Refinement
pageTutorial: Mask CreationpageCase Study: Yeast U4/U6.U5 tri-snRNPConformational Variability
pageTutorial: 3D Classification (BETA)pageTutorial: 3D Variability Analysis (Part One)pageTutorial: 3D Variability Analysis (Part Two)pageTutorial: 3D Flexible RefinementpageTutorial: 3D Flex Mesh PreparationLast updated