CryoSPARC Guide
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Data Processing Tutorials

Tutorials on how to use CryoSPARC jobs and processing cryo-EM data.

Introduction to CryoSPARC

Case Studies

Data Processing

Alongside these tutorials, see: All Job Types in CryoSPARC and Tutorial Videos

Files, Data and Import

Preprocessing

Particle Picking

Ab-initio Reconstruction

Case Study: Previously unknown conformation of AAA+ unfoldase discovered using cryoSPARC

Helical Reconstruction

3D Refinement

Local Refinement

Conformational Variability