Tutorial Videos
June 10-12, 2020
CryoSPARC was presented at this multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Please see links to the recordings below, courtesy of S2C2.
What's covered:
Documentation, user support, user interface overview
T20S tutorial (import of raw movies through to final refinement)
Audience Q&A throughout
Watch directly on YouTube to access timestamps (expand the video description):
What's covered:
Processing of the HA Trimer (EMPIAR-10097) dataset
Nav1.7 Channel (EMPIAR-10261) case study
Processing of AAA+ unfoldase data
Processing of apoferritin dataset
Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset
Non-uniform refinement
Processing of ATPase dataset
Conformational heterogeneity and 3D Variability Analysis
Audience Q&A throughout
Watch directly on YouTube to access detailed descriptions and timestamps.
Watch directly on YouTube to access detailed descriptions and timestamps.
June 30, 2020
Learn how the new 3D Variability Analysis (3DVA) algorithm in cryoSPARC can reveal new functional and biological insight into the conformational and flexible motion dynamics of a protein molecule from single particle cryo-EM data.
We will cover the new concepts introduced by the algorithm, interpretation of results, several case studies and examples, practical considerations to keep in mind when working on your own data, and live audience Q&A. 3DVA has already been used in many notable structural studies to shed light on protein dynamics, including GPCR structures and the SARS-CoV-2 spike protein. To read ahead, see the 3DVA preprint.
May 1, 2019
Summary: Educational webinar on how cryo-EM offers value for drug discovery and structure-based drug design, on targets like GPCRs and membrane proteins
Topics covered: why is cryo-EM useful for drug discovery; computational aspects involved in cryo-EM structure determination; future advancements