Stanford-SLAC Cryo-EM Center (S2C2) CryoEM Image Processing Workshop (June 10-12, 2020)

CryoSPARC was presented at this multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Please see links to the recordings below, courtesy of S2C2. The workshop agenda, slides and original video links can be found on the S2C2 CryoEM Image Processing Workshop page:

Day 1 - CryoSPARC introduction and basic processing (T20S)

What's covered:

• Documentation, user support, user interface overview

• T20S tutorial (import of raw movies through to final refinement)

• Audience Q&A throughout

​Watch directly on YouTube to access timestamps (expand the video description):

Day 2 - Advanced processing (multiple datasets)

What's covered:

• Processing of the HA Trimer (EMPIAR-10097) dataset

• Nav1.7 Channel (EMPIAR-10261) case study

• Processing of AAA+ unfoldase data

• Processing of apoferritin dataset

• Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset

• Non-uniform refinement

• Processing of ATPase dataset

• Conformational heterogeneity and 3D Variability Analysis

• Audience Q&A throughout

Part 1

​Watch directly on YouTube to access detailed descriptions and timestamps.

Part 2

​Watch directly on YouTube to access detailed descriptions and timestamps.

Resolving flexibility and heterogeneity with 3D Variability Analysis

Learn how the new 3D Variability Analysis (3DVA) algorithm in cryoSPARC can reveal new functional and biological insight into the conformational and flexible motion dynamics of a protein molecule from single particle cryo-EM data.

We will cover the new concepts introduced by the algorithm, interpretation of results, several case studies and examples, practical considerations to keep in mind when working on your own data, and live audience Q&A. 3DVA has already been used in many notable structural studies to shed light on protein dynamics, including GPCR structures and the SARS-CoV-2 spike protein. To read ahead, see the 3DVA preprint.