Tutorial Videos

CryoSPARC tutorial videos.

For written tutorials, please see:

Data Processing TutorialsCryoSPARC Guide

CryoSPARC Basic Processing

S2C2 CryoEM Image Processing Workshop 2020 (Day 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.

What's covered:

• Documentation, user support, user interface overview

• T20S tutorial (import of raw movies through to final refinement)

• Audience Q&A throughout

Watch directly on YouTube to access timestamps (expand the video description):

CryoSPARC Advanced Processing (2-part video)

What's covered:

• Processing of the HA Trimer (EMPIAR-10097) dataset

• Nav1.7 Channel (EMPIAR-10261) case study

• Processing of AAA+ unfoldase data

• Processing of apoferritin dataset

• Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset

• Non-uniform refinement

• Processing of ATPase dataset

• Conformational heterogeneity and 3D Variability Analysis

• Audience Q&A throughout

Part 1 of 2

S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.

Part 1

Watch directly on YouTube to access detailed descriptions and timestamps.

Part 2 of 2

S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 2). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.

Part 2

Watch directly on YouTube to access detailed descriptions and timestamps.

CryoSPARC Live Walkthrough

Processing EMPIAR-10288 using CryoSPARC Live.

For a detailed guide on CryoSPARC Live, see:

New Live Session: Start to Finish Guide

Reference Based Motion Correction

How to use Reference Based Motion Correction in CryoSPARC v4.4+.

Workflows

How to construct an automated pipeline of jobs in v4.4+.

Mask Creation in ChimeraX

Creating a mask in ChimeraX using three different techniques: volume segmentation, volume eraser and molmap.

3D Flex Custom Mesh Generation

When might your data benefit from a custom mesh, and the process of mesh creation.