CryoSPARC Guide
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Setup, Configuration and Management
Deployment Guides
Processing Data in cryoSPARC
Real-time processing in cryoSPARC Live
Cryo-EM Data Processing Explained
Tutorial Videos and Webinars
Links to cryoSPARC tutorial videos.
Tutorial Video: CryoSPARC Basic Processing
June 2020
S2C2 CryoEM Image Processing Workshop 2020 (Day 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.
What's covered
- Documentation, user support, user interface overview
- T20S tutorial (import of raw movies through to final refinement)
- Audience Q&A throughout
Tutorial Video: CryoSPARC Advanced Processing (2-part video)
June 2020
What's covered
- Processing of the HA Trimer (EMPIAR-10097) dataset
- Nav1.7 Channel (EMPIAR-10261) case study
- Processing of AAA+ unfoldase data
- Processing of apoferritin dataset
- Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset
- Non-uniform refinement
- Processing of ATPase dataset
- Conformational heterogeneity and 3D Variability Analysis
- Audience Q&A throughout
Video 1 of 2
S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.
Part 1
Video 2 of 2
S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 2). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.
Part 2
Webinar: Real-time cryo-EM analysis for all: cryoSPARC Live
June 2021
Learn how cryoSPARC Live, a seamless real-time 2D and 3D processing system for single particle cryo-EM, accelerates time-to-structure and drives rapid insights into sample characteristics and data quality, enabling decision making while the sample is still in the microscope.
CryoSPARC Live is not just for facilities; it is also the fastest, simplest way for beginners and experts alike to process cryo-EM data that has already been collected. We will cover use cases, performance considerations, real-time experimentation and practical workflows, and will be joined by two expert guest speakers, Giovanna Scapin (NIS) and Craig Yoshioka (PNCC), who will discuss how they use cryoSPARC Live in practice in both industry and academic settings.
Tutorial Video: CryoSPARC Live 10-Minute Overview
June 2021
For more information about starting your own Session in cryoSPARC Live, see:
Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis
June 2020
Learn how the new 3D Variability Analysis (3DVA) algorithm in cryoSPARC can reveal new functional and biological insight into the conformational and flexible motion dynamics of a protein molecule from single particle cryo-EM data.
We will cover the new concepts introduced by the algorithm, interpretation of results, several case studies and examples, practical considerations to keep in mind when working on your own data, and live audience Q&A. 3DVA has already been used in many notable structural studies to shed light on protein dynamics, including GPCR structures and the SARS-CoV-2 spike protein. To read ahead, see the 3DVA preprint.
3D Variability Analysis Webinar hosted on June 30, 2020
Webinar: CryoEM for Drug Discovery
May 2019
Summary: Educational webinar on how cryo-EM offers value for drug discovery and structure-based drug design, on targets like GPCRs and membrane proteins
Topics covered: why is cryo-EM useful for drug discovery; computational aspects involved in cryo-EM structure determination; future advancements
Last modified 1mo ago
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Contents
Tutorial Video: CryoSPARC Basic Processing
What's covered
Tutorial Video: CryoSPARC Advanced Processing (2-part video)
What's covered
Video 1 of 2
Video 2 of 2
Webinar: Real-time cryo-EM analysis for all: cryoSPARC Live
Tutorial Video: CryoSPARC Live 10-Minute Overview
Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis
Webinar: CryoEM for Drug Discovery