Tutorial Videos

CryoSPARC: Basic and Advanced Processing
June 10-12, 2020
CryoSPARC was presented at this multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Please see links to the recordings below, courtesy of S2C2.
Introduction and basic processing (Day 1)
What's covered:
Documentation, user support, user interface overview
T20S tutorial (import of raw movies through to final refinement)
Audience Q&A throughout
​Watch directly on YouTube to access timestamps (expand the video description):  
S2C2 CryoEM Image Processing Workshop 2020 (Day 1)
Advanced processing (multiple datasets) (Day 2)
What's covered:
Processing of the HA Trimer (EMPIAR-10097) dataset
Nav1.7 Channel (EMPIAR-10261) case study
Processing of AAA+ unfoldase data
Processing of apoferritin dataset
Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset
Non-uniform refinement
Processing of ATPase dataset
Conformational heterogeneity and 3D Variability Analysis
Audience Q&A throughout
Part 1
​Watch directly on YouTube to access detailed descriptions and timestamps.
S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 1)
Part 2
​Watch directly on YouTube to access detailed descriptions and timestamps.
S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 2)
Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis
June 30, 2020
Learn how the new 3D Variability Analysis (3DVA) algorithm in cryoSPARC can reveal new functional and biological insight into the conformational and flexible motion dynamics of a protein molecule from single particle cryo-EM data.
We will cover the new concepts introduced by the algorithm, interpretation of results, several case studies and examples, practical considerations to keep in mind when working on your own data, and live audience Q&A. 3DVA has already been used in many notable structural studies to shed light on protein dynamics, including GPCR structures and the SARS-CoV-2 spike protein. To read ahead, see the 3DVA preprint.
3D Variability Analysis Webinar hosted on June 30, 2020
Webinar: CryoEM for Drug Discovery
May 1, 2019
Summary: Educational webinar on how cryo-EM offers value for drug discovery and structure-based drug design, on targets like GPCRs and membrane proteins
Topics covered: why is cryo-EM useful for drug discovery; computational aspects involved in cryo-EM structure determination; future advancements
Webinar: Real-time cryo-EM analysis for all: cryoSPARC Live
Learn how cryoSPARC Live, a seamless real-time 2D and 3D processing system for single particle cryo-EM, accelerates time-to-structure and drives rapid insights into sample characteristics and data quality, enabling decision making while the sample is still in the microscope.
CryoSPARC Live is not just for facilities; it is also the fastest, simplest way for beginners and experts alike to process cryo-EM data that has already been collected. We will cover use cases, performance considerations, real-time experimentation and practical workflows, and will be joined by two expert guest speakers, Giovanna Scapin (NIS) and Craig Yoshioka (PNCC), who will discuss how they use cryoSPARC Live in practice in both industry and academic settings.
cryoSPARC Live: 10-Minute Overview
For more information about starting your own Session in cryoSPARC Live, see:
New Live Session: Start to Finish Guide
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Tutorials and Case Studies

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Contents

CryoSPARC: Basic and Advanced Processing

Introduction and basic processing (Day 1)

Advanced processing (multiple datasets) (Day 2)

Webinar: Resolving flexibility and heterogeneity with 3D Variability Analysis

Webinar: CryoEM for Drug Discovery

Webinar: Real-time cryo-EM analysis for all: cryoSPARC Live

cryoSPARC Live: 10-Minute Overview