CryoSPARC Guide
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Tutorial Videos

CryoSPARC tutorial videos.
For written tutorials, please see:

1) CryoSPARC Basic Processing

June 2020
S2C2 CryoEM Image Processing Workshop 2020 (Day 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.
What's covered:
  • Documentation, user support, user interface overview
  • T20S tutorial (import of raw movies through to final refinement)
  • Audience Q&A throughout
Watch directly on YouTube to access timestamps (expand the video description):

2) CryoSPARC Advanced Processing (2-part video)

June 2020
What's covered:
  • Processing of the HA Trimer (EMPIAR-10097) dataset
  • Nav1.7 Channel (EMPIAR-10261) case study
  • Processing of AAA+ unfoldase data
  • Processing of apoferritin dataset
  • Processing of the Cannabinoid Receptor 1-G Protein Complex (EMPIAR-10288) dataset
  • Non-uniform refinement
  • Processing of ATPase dataset
  • Conformational heterogeneity and 3D Variability Analysis
  • Audience Q&A throughout

Part 1 of 2

S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 1). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.

Part 1

Watch directly on YouTube to access detailed descriptions and timestamps.

Part 2 of 2

S2C2 CryoEM Image Processing Workshop 2020 (Day 2 Part 2). CryoSPARC was presented at multi-day virtual workshop hosted by the Stanford-SLAC Cryo-EM Center (S2C2). Recordings courtesy of S2C2.

Part 2

Watch directly on YouTube to access detailed descriptions and timestamps.

3) CryoSPARC Live 10-Minute Overview

June 2021
A 10-minute primer on CryoSPARC Live capabilities.
For a detailed guide on CryoSPARC Live, see:

4) CryoSPARC Live Walkthrough

Dec 2020
Processing EMPIAR-10288 using CryoSPARC Live.