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Tutorial: BILD files in CryoSPARC v4.4+
An overview of BILD files in CryoSPARC
BILD files are now produced for all viewing direction distribution figures in CryoSPARC v4.4+.
Prior to CryoSPARC v4.4., a standard way to construct BILD files from CryoSPARC refinement jobs required the use of the
star2bild.pyscripts within the external open-source package pyem. To make this process easier, CryoSPARC v4.4 now includes BILD files with every viewing direction distribution figure in the event log.
To use these files, click the
[bild]link on top of any viewing direction figure to download the BILD file:
UCSF Chimera X window with a refined map and an overlayed viewing distribution
star2bild.py, we generate BILD files by creating spherical bins using the HealPix algorithm. Each spherical bin is visualized as a cylinder whose base is on a sphere with radius
is the box size and
is the pixel size in Angstroms. Cylinder height is scaled linearly with relative particle population size, ranging from 0 (displayed as flat blue circle) to
(displayed as a red cylinder).