FAQs and Troubleshooting
CryoSPARC Live is built for anyone with access to a microscope or cryo-EM single particle data:
- Data collection facilities, cryo-EM cores and microscope operators who want to make the most of microscope time with real-time data quality assessment
- Individual users who want to gain insights about data quality by performing 3D reconstructions in real-time with data collection
- Users who are looking for the simplest, fastest way to process previously collected data in a seamless and "first cut" manner
CryoSPARC Live is available free of charge for academic use.
CryoSPARC Live jobs are housed within CryoSPARC projects, so there is full integration between the two applications and you can continue processing in CryoSPARC using the outputs of Live.
CryoSPARC Live supports reading in movie files (
.tif
, .mrc
, .mrc.bz2
and .eer
). For more information about EER file support in CryoSPARC, please see: EER File SupportCryoSPARC Live can use multiple GPUs concurrently for pre-processing and reconstruction jobs to maximize achievable throughput. With 4 GPUs assigned to preprocessing (and sufficiently fast storage), cryoSPARC Live can sustain a rate of up to 1 exposure per 1.4 seconds, or over 60,000 exposures per 24-hours. For more performance metrics, please see: Performance Metrics