Job: Import Particle Stack
- Import a stack of particles with metadata and CTF parameters.
- .star, .mrc, .txt, .par, and/or .emx
- It is best to specify the
Raw pixel size (A),
Accelerating voltage (kV),
Spherical aberration (mm)and
Total exposure dose (e/A^2)if known
Data Sign:[dark-on-light] +1 for dark particles on light background, [light-on-dark] -1 for light particles on dark background. Cryo-EM data is typically recorded as +1 but is often inverted during processing, negative stain EM data is typically recorded as -1'
- Imported particles
- 2D Classification to sort and classify particles and remove "junk" particles. Or, if the particle stack is already "clean", ab-initio reconstruction can be used to generate one or more initial models which can then be refined