# Job: 3D Flexible Refinement (3DFlex) (BETA)

{% hint style="info" %}
Currently, 3D Flex Refinement requires that the input particle images have a box size no larger than 440 pixels.
{% endhint %}

3D Flexible Refinement (3DFlex) is a motion-based deep generative model for continuous heterogeneity. It can model non-rigid motion and flexibility of a protein molecule across its conformational landscape, and can use the motion model to combine signal from particle images in different conformations to improve refinement resolution in flexible regions.

For more details on this job, available in CryoSPARC v4.1+, please see:

{% content-ref url="../../tutorials-and-case-studies/tutorial-3d-flexible-refinement" %}
[tutorial-3d-flexible-refinement](https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/tutorial-3d-flexible-refinement)
{% endcontent-ref %}

{% content-ref url="../../tutorials-and-case-studies/tutorial-3d-flex-mesh-preparation" %}
[tutorial-3d-flex-mesh-preparation](https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/tutorial-3d-flex-mesh-preparation)
{% endcontent-ref %}


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# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/variability/job-3d-flexible-refinement-3dflex-beta.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.