> For the complete documentation index, see [llms.txt](https://guide.cryosparc.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/variability/job-3d-flexible-refinement-3dflex-beta.md).

# Job: 3D Flexible Refinement (3DFlex) (BETA)

{% hint style="info" %}
Currently, 3D Flex Refinement requires that the input particle images have a box size no larger than 440 pixels.
{% endhint %}

3D Flexible Refinement (3DFlex) is a motion-based deep generative model for continuous heterogeneity. It can model non-rigid motion and flexibility of a protein molecule across its conformational landscape, and can use the motion model to combine signal from particle images in different conformations to improve refinement resolution in flexible regions.

For more details on this job, available in CryoSPARC v4.1+, please see:

{% content-ref url="/pages/tJUJijPRuOI8t3kB0MNV" %}
[Tutorial: 3D Flexible Refinement](/processing-data/tutorials-and-case-studies/tutorial-3d-flexible-refinement.md)
{% endcontent-ref %}

{% content-ref url="/pages/63kKkK7nvLpPDmOx6PEI" %}
[Tutorial: 3D Flex Mesh Preparation](/processing-data/tutorials-and-case-studies/tutorial-3d-flex-mesh-preparation.md)
{% endcontent-ref %}


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://guide.cryosparc.com/processing-data/all-job-types-in-cryosparc/variability/job-3d-flexible-refinement-3dflex-beta.md?ask=<question>&goal=<endgoal>
```

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