Job: Homogeneous Reconstruction Only

Description

Create a 3D reconstruction from input particles that already have alignments in 3D. Two reconstructions are created based on the split of particles into half-maps either from the input, or by resplitting (as an option). The half-maps are reconstructed the same way as they would be in a refinement job, but no alignment iterations are performed.

The input mask (which is required) is used for computing FSC between the reconstructed half-maps.

The output of this job can be used with the FSC Validation job, or with the sharpening job, or any other that requires volumes and half-maps.

Input

  • Particles (with alignments3D)

  • Mask

Common Parameters

Output:

  • Reconstructed half-maps

  • Gold-standard FSC curve

Common Next Steps

  • Download and inspect map