CryoSPARC Guide
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CryoSPARC Guide
About cryoSPARC
Current Version
Licensing
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cryoSPARC Installation Prerequisites
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Deploying cryoSPARC on AWS
Processing Data in cryoSPARC
Cryo-EM Data Processing in cryoSPARC: Introductory Tutorial
User Interface and Usage Guide
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Particle Curation
3D Reconstruction
3D Refinement
Job: Homogeneous Refinement (Legacy)
Job: Homogeneous Refinement
Job: Heterogeneous Refinement
Job: Non-uniform Refinement (Legacy)
Job: Non-Uniform Refinement (NEW)
Job: Homogeneous Reconstruction Only
CTF Refinement
Variability
Postprocessing
Local Refinement
Helical Reconstruction (BETA)
Utilities
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Real-time processing in cryoSPARC Live
About cryoSPARC Live
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UI Overview
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New Live Session: Start to Finish Guide
Tutorial Videos
Performance Metrics
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Cryo-EM Data Processing Explained
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cryoSPARC Discussion Forum
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Job: Homogeneous Refinement

Description

Homogeneous Refinement (as differentiated from Homogeneous Refinement (Legacy)) provides support for higher-order aberration (beam tilt, spherical aberration, trefoil, tetrafoil) correction and per-particle defocus refinement on the fly.
As of cryoSPARC v3.3, Homogeneous Refinement also supports anisotropic magnification and Ewald Sphere correction.
Please see:
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Job: Homogeneous Refinement (Legacy)
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Job: Heterogeneous Refinement
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