Interactive Job: Manual Picker
Manual picking.
The information in this section applies to CryoSPARC ≤v3.3. For CryoSPARC v4.0+, please see:Interactive Jobs
- Manually pick particles interactively from micrographs.
- Aligned/motion-corrected micrographs
- Once the job is running, you can input and adjust the
Box sizein the job inspector/stream log. We recommend a box size that is at least double the diameter of the particle. - You can also adjust the
Contrast Intensity Overrideslider to manually select the contrast bounds when viewing a micrograph.
- Particles
When manually picking particles with the goal of creating a template for auto-picking, we recommend picking particles from multiple micrographs with a range of defocus and CTF fit values to ensure a comprehensive template.
Manual picking is the most time consuming picking method. As such, this job is recommended for picking a small subset of micrographs for the purposes of providing initial picks to template picking and/or deep picking instead of picking entire datasets.
- 2D Classification and Select 2D to generate templates for Template picking
Last modified 1mo ago