# Job: 3D Variability Display

## **Description**

Create various versions of a 3D variability result that can be used for display. Read a tutorial [here](https://guide.cryosparc.com/processing-data/tutorials-and-case-studies/tutorial-3d-variability-analysis-part-one).

## **Input**

* Particles (from 3D Variability Analysis)
* Volume (from 3D Variability Analysis)

## **Common Parameters**

* Output mode**:** 
  * **simple mode**: output a simple linear "movie" of volumes along each dimension. Number of frames is the next param. 
  * **cluster mode**: fit clusters to reaction coordinates and output volumes and particles from each cluster. Number of clusters is the next param. 
  * **intermediates mode:** reconstruct multiple intermediate volumes along each variability dimension, useful for better visualizing non-linear changes. Number of intermediate frames is the next param. 
* Intermediates: window (frames):
  * Size of rolling window in each bin (in number of frames). Set to a positive integer to define a triangular weighting function for each volume. Set to 0 for a tophat weighting function. Set to -1 to create bins with equal number of particles.

## **Output**

* Volume series of each variability component
* \[Optionally] particles for each cluster or intermediate frame 
* \[Optionally] volume for each cluster or intermediate frame

## **Common Next Steps**

* Visualization of results through UCSF Chimera or similar.