CryoSPARC Guide

Job: Sharpening Tools

Re-sharpen and adjust the B-factor of a volume after refinement.


  • To produce optimal results in publications and for model-building, it is often necessary to re-sharpen and adjust the B-factor of a volume after completing a refinement. This is done by the Sharpening Tools job.


  • A map volume
    • map_half_A
    • map_half_B
  • A mask


  • A sharpened map
    • map_sharp
  • A mask
    • mask_fsc

Common Parameters

  • B-Factor to apply: A negative value. A general rule of thumb is to start with the B-Factor value presented in the Guinier Plot of a refinement job (e.g. -76.7)

Common Next Steps

  • Downloading and inspecting the sharpened map in a software such as UCSF Chimera
Download options for the "volume" output group in Sharpening Tools