Job: Sharpening Tools
Re-sharpen and adjust the B-factor of a volume after refinement.
- To produce optimal results in publications and for model-building, it is often necessary to re-sharpen and adjust the B-factor of a volume after completing a refinement. This is done by the Sharpening Tools job.
- A map volume
map_half_Amap_half_B
- A mask
- A sharpened map
map_sharp
- A mask
mask_fsc
B-Factor to apply: A negative value. A general rule of thumb is to start with the B-Factor value presented in the Guinier Plot of a refinement job (e.g. -76.7)
- Downloading and inspecting the sharpened map in a software such as UCSF Chimera
Download options for the "volume" output group in Sharpening Tools
Last modified 1yr ago