Job: Sharpening Tools

Re-sharpen and adjust the B-factor of a volume after refinement.

Description

• To produce optimal results in publications and for model-building, it is often necessary to re-sharpen and adjust the B-factor of a volume after completing a refinement. This is done by the Sharpening Tools job.

Input

• A map volume

  • map_half_A

  • map_half_B

• A mask

Output

• A sharpened map

  • map_sharp

• A mask

  • mask_fsc

Common Parameters

• B-Factor to apply: A negative value. A general rule of thumb is to start with the B-Factor value presented in the Guinier Plot of a refinement job (e.g. -76.7)

Common Next Steps

• Downloading and inspecting the sharpened map in a software such as UCSF Chimera

Download options for the "volume" output group in Sharpening Tools