CryoSPARC Guide
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CryoSPARC Guide
About cryoSPARC
Current Version
Licensing
Setup, Configuration and Management
Hardware and System Requirements
cryoSPARC Installation Prerequisites
How to Download, Install and Configure
Software Updates
Management and Monitoring
Performance Metrics
Troubleshooting
Deployment Guides
Deploying cryoSPARC on AWS
Processing Data in cryoSPARC
Cryo-EM Data Processing in cryoSPARC: Introductory Tutorial
User Interface and Usage Guide
All Job Types in cryoSPARC
Workflows
Import
Motion Correction
CTF Estimation
Exposure Curation
Particle Picking
Interactive Job: Manual Picker
Job: Blob Picker
Job: Template Picker
Job: Filament Tracer (BETA)
Job: Blob Picker Tuner
Interactive Job: Inspect Particle Picks
Job: Extract from Micrographs
Job: Extract from Micrographs (CPU)
Job: Downsample Particles
Deep Picking
Particle Curation
3D Reconstruction
3D Refinement
CTF Refinement
Variability
Postprocessing
Local Refinement
Helical Reconstruction (BETA)
Utilities
Simulations
Tutorial Videos and Webinars
Tutorials and Usage Guides
Real-time processing in cryoSPARC Live
About cryoSPARC Live
Prerequisites and Compute Resources Setup
How to Access cryoSPARC Live
UI Overview
Live Jobs and Session-Level Functions
New Live Session: Start to Finish Guide
Tutorial Videos
Performance Metrics
FAQs and Troubleshooting
Cryo-EM Data Processing Explained
Questions and Support
cryoSPARC Discussion Forum
cryoSPARC Blog
cryoSPARC Homepage
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Job: Template Picker

Description

  • Auto-pick particles from micrographs using a template generated from 2D classification in cryoSPARC, or from a template imported from elsewhere.

Input

  • One or more templates (generated in cryoSPARC or elsewhere)
  • Aligned/motion-corrected micrographs

Common Parameters

  • Particle diameter in Angstrom:
  • Minimum distance between particles:

Output

  • Particles

Common Next Steps

  • Inspect picks
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Job: Blob Picker
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Job: Filament Tracer (BETA)
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Contents
Description
Input
Common Parameters
Output
Common Next Steps