.mrc, .mrc.bz2 , .tif or .eer formats..eer file support, please see:Movies data path: Click on the folder icon to browse or paste the path specifying the location where the movies are stored. To select multiple files, enter a wildcard expression in the browse bar, e.g., *.mrc, which will select all matching file types in the subfolder.Gain reference path: Specify the path to the gain reference file, if available.Raw pixel size (A), Accelerating voltage (kV), Spherical aberration (mm) and Total exposure dose (e/A^2) if knownNegative Stain Data: If Negative Stain Data is on, this indicates that there are light particles on dark background (-1). If it's off, this indicates the movies have dark particles on light background (cryo-em data, +1).Phase Plate Data: Indicates if data was collected using a phase plate.