Job: Import Movies
Import raw movies.
- Import one or more raw movies for processing.
- Raw movies in
.eerfile support, please see:
If working with negative stain or phase plate data, please see:
Movies data path:Click on the folder icon to browse or paste the path specifying the location where the movies are stored. To select multiple files, enter a wildcard expression in the browse bar, e.g., *.mrc, which will select all matching file types in the subfolder.
Gain reference path:Specify the path to the gain reference file, if available.
- It is best to specify the
Raw pixel size (A),
Accelerating voltage (kV),
Spherical aberration (mm)and
Total exposure dose (e/A^2)if known
Negative Stain Data:If Negative Stain Data is on, this indicates that there are light particles on dark background (-1). If it's off, this indicates the movies have dark particles on light background (cryo-em data, +1).
Phase Plate Data:Indicates if data was collected using a phase plate.
- Imported movies (which can be used in other CryoSPARC jobs)
- Full frame motion correction to generate micrographs from which particles can be picked manually or using template-based automatic picking