Job: Import Movies

Description
Import one or more raw movies for processing.
Input
Raw movies in .mrc, .mrc.bz2 , .tif or .eer formats.
For .eer file support, please see:
Tutorial: EER File Support
If working with negative stain or phase plate data, please see:
Tutorial: Negative Stain Data
Tutorial: Phase Plate Data
Common Parameters
Movies data path: Click on the folder icon to browse or paste the path specifying the location where the movies are stored. To select multiple files, enter a wildcard expression in the browse bar, e.g., *.mrc, which will select all matching file types in the subfolder.
Gain reference path: Specify the path to the gain reference file, if available.
It is best to specify the Raw pixel size (A), Accelerating voltage (kV), Spherical aberration (mm) and Total exposure dose (e/A^2) if known
Negative Stain Data: If Negative Stain Data is on, this indicates that there are light particles on dark background (-1). If it's off, this indicates the movies have dark particles on light background (cryo-em data, +1).
Phase Plate Data: Indicates if data was collected using a phase plate.
Output
Imported movies (which can be used in other cryoSPARC jobs)
Common Next Steps
Full frame motion correction to generate micrographs from which particles can be picked manually or using template-based automatic picking

Import

Job: Import Micrographs

Last modified 6mo ago

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