Case Study: Exploratory data processing by Oliver Clarke
Exploratory data processing in CryoSPARC workshop notes made available by Oliver Clarke, PhD, Assistant Professor at Columbia University (June 2023).
This workshop is intended to provide an introduction to "exploratory" data processing in CryoSPARC - that is, data processing with the goal of quickly identifying, reconstructing and refining the molecular species present in a heterogeneous sample. CryoSPARC is used here, but the same general principles & workflow apply to single particle processing in any software package (the particles and micrographs should be directly importable into RELION - just convert the particle.cs file to a STAR file using csparc2star.py in pyem ( see here ) and you should be good to go).
Please refer to the detailed workshop notes here:
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