Limitations: The Blob Picker Tuner can not discriminate particles better than the regular Blob Picker, but makes the process of identifying useful parameters much easier. For the Blob Picker Tuner, the number of labelled particles is important. Labelling too many particles may introduce bias into the Blob Picker based on the human bias of picking. On the other hand, labelling too few particles may result in poor picks. Somewhere from 15-40 particles picks is recommended, but more may be needed for difficult datasets. Neighbouring particles should be picked for manual input to ensure the tuner has information about particle spacing. Read more in the tutorial, here.