Job: Align 3D maps
Align two or more 3D maps.
Description
Use this tool to align two or more 3D maps to a common reference in order to better analyze maps obtained from different experiments. The maps do not need to come from the same type of job. For example, one could align a coarse resolution map obtained from ab-initio reconstruction with a higher resolution map obtained from refinement.
Input
Reference Map
Map(s) to Align
(Optionally) Particles
Output
Reference Map
Aligned Map(s)
(Optionally) Particles with updated
alignments3D
Common Parameters
Update particle alignments
will enable the option to update particlealignments3D
values based on the computed 3D rigid body transform of each alignment. If this is turned on, additional particle inputs will be generated for all maps to be aligned.Mask threshold factor
should be set to a value between 0 and 1. This value will be used to create a mask around the density to exclude solvent during alignment.Log rotation matrices, figures and difference maps
will produce verbose output for the job.
Common Next Steps
Local Refinement
Homogeneous Reconstruction Only (to check that particle alignments are correct)
Last updated