Job: Align 3D maps
Align two or more 3D maps.
Use this tool to align two or more 3D maps to a common reference in order to better analyze maps obtained from different experiments. The maps do not need to come from the same type of job. For example, one could align a coarse resolution map obtained from ab-initio reconstruction with a higher resolution map obtained from refinement.
- Reference Map
- Map(s) to Align
- (Optionally) Particles
- Reference Map
- Aligned Map(s)
- (Optionally) Particles with updated
alignments3D
Update particle alignmentswill enable the option to update particlealignments3Dvalues based on the computed 3D rigid body transform of each alignment. If this is turned on, additional particle inputs will be generated for all maps to be aligned.Mask threshold factorshould be set to a value between 0 and 1. This value will be used to create a mask around the density to exclude solvent during alignment.Log rotation matrices, figures and difference mapswill produce verbose output for the job.
- Local Refinement
- Homogeneous Reconstruction Only (to check that particle alignments are correct)
Last modified 6mo ago