CryoSPARC Live Tutorial Videos
Last updated
Last updated
Join us while we process cryo-EM data (EMPIAR-10288) using cryoSPARC Live. We'll walk you through the interface and process this dataset from start to finish.
CryoSPARC Live is available with the cryoSPARC software platform:
How to Access cryoSPARC LiveFor more information about starting your own Session in cryoSPARC Live, see:
New Live Session: Start to Finish GuideDownload EMPIAR-10288, the dataset used in this walkthrough:
The gain reference file provided in this dataset is in a file format (.dm4) that cryoSPARC doesn't support. Click this link to download a compatible version of the gain reference to use in cryoSPARC.
Microscope/Camera
Raw pixel size (Å): 0.86
Accelerating voltage (kV): 300
Spherical aberration (mm): 2.7
Total exposure dose (e/Å^2): 58
Advanced: Flip gain ref in Y? : True
Blob Picker
Minimum particle diameter (Å): 100
Maximum particle diameter (Å): 150
Advanced: Use elliptical blob: True
Extraction box size (pix): 320
Advanced: Fourier crop to box size (pix): 240
Learn how cryoSPARC Live, a seamless real-time 2D and 3D processing system for single particle cryo-EM, accelerates time-to-structure and drives rapid insights into sample characteristics and data quality, enabling decision making while the sample is still in the microscope.
CryoSPARC Live is not just for facilities; it is also the fastest, simplest way for beginners and experts alike to process cryo-EM data that has already been collected. We will cover use cases, performance considerations, real-time experimentation and practical workflows, and will be joined by two expert guest speakers, Giovanna Scapin (NIS) and Craig Yoshioka (PNCC), who will discuss how they use cryoSPARC Live in practice in both industry and academic settings.
For more information about starting your own Session in cryoSPARC Live, see:
New Live Session: Start to Finish Guide