blob
, ctf
, and alignments3D
)map
, or map_half_A
and map_half_B
)Threshold
and Soft padding width
parameters. We typically recommend setting the Soft padding width
parameter to be a voxel value that ensures the resulting padding is several factors larger than the expected resolution of the complex. A good rule of thumb gives the minimum mask softness as 5 * res_angstroms / pixel_size_angstroms.
For example, a complex reaching 3.0Â Ã… in resolution with a pixel size of 1.0 Ã… should be padded to at least 5 * 3.0 / 1.0 = 15 voxels. Number of particles to subtract:
The number of signal-subtracted particles to output. Runtime will be faster for fewer particles. If left as None, signal subtraction will be done to all particlesLow-pass Filter Input Structure (Ã…):
Resolution (in Angstroms) corresponding to the low-pass filter applied to the volume.mrc
) will be outputted in smaller batches corresponding to the memory available in the GPU, but a single .cs
file will contain the metadata for the entire stack. Make sure you have enough space on disk before you run this job!Cache particle images to SSD
as true will store particle images on the SSD for faster processing. This requires adequate space on the SSD, the amount of which is determined at runtime.