Soft padding widthparameters. We typically recommend setting the
Soft padding widthparameter to be a voxel value that ensures the resulting padding is several factors larger than the expected resolution of the complex. A good rule of thumb gives the minimum mask softness as
5 * res_angstroms / pixel_size_angstroms.For example, a complex reaching 3.0 Å in resolution with a pixel size of 1.0 Å should be padded to at least 5 * 3.0 / 1.0 = 15 voxels.
Number of particles to subtract:The number of signal-subtracted particles to output. Runtime will be faster for fewer particles. If left as None, signal subtraction will be done to all particles
Low-pass Filter Input Structure (Å):Resolution (in Angstroms) corresponding to the low-pass filter applied to the volume
.mrc) will be outputted in smaller batches corresponding to the memory available in the GPU, but a single
.csfile will contain the metadata for the entire stack. Make sure you have enough space on disk before you run this job!
Cache particle images to SSDas true will store particle images on the SSD for faster processing. This requires adequate space on the SSD, the amount of which is determined at runtime.