CryoSPARC Guide

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Setup, Configuration and Management

Deployment Guides

Processing Data in cryoSPARC

Real-time processing in cryoSPARC Live

Cryo-EM Data Processing Explained

Job: Particle Sets Tool

Description

Split a particle group into subsets or compare two groups.

`particles_A`

: Particles- With only this group$A$specified, choose the
`split`

action to split the data into multiple batches

`particles_B`

: Particles (optional)- With both groups$A$and$B$, choose the
`intersect`

action to compute set operations on the two groups, including (i) intersection$A âˆ© B$and differences (ii)$A - B$and (iii)$B - A$.

`Action`

- With
*only one particle input group*, chooseto split the data into multiple batches`split`

- With
*both particle input groups*, chooseto compute the set intersection and difference between the two groups.`intersect`

- if
`intersect`

is selected, a new parameter is available:`Field to Intersect`

`uid`

: use the`uid`

field to intersect the two inputs`path`

: use a common path field to intersect the two inputs (e.g.,`location/micrograph_path`

)

`Split num. batches`

: With the`split`

action, how many batches to split the particles into`Split batch size`

: With the`split`

action, how many particles to add to each batch`Split randomize`

: With the`split`

action, randomize the particles to add to each batch

`split_X`

(multiple outputs): With the`split`

action, batches of the resulting particles`remainder`

: With the`split`

action, particles left out of`split_X`

batches due to the specified batch size and number of batches`intersect`

: With the`intersect`

action, result of$A âˆ© B$â€‹`A_minus_B`

: With the`intersect`

action, set difference$A - B$â€‹`B_minus_A`

: With the`intersect`

action, set difference$B - A$â€‹

Example outputs when only one input particle set is provided compared to two sets.

Common Next Steps

- 2D Classification

Last modified 8mo ago

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