Downsample particles and save them with smaller box sizes and optionally apply filters.
Downsample particles outputs depicting original and processed particles
Particles (blob required; alignments2D and alignments3D optional)
Crop / pad to box size (pix): Crop in real space to this size before downsampling. If this number is larger than the input size, each particle will be padded with its mean pixel value
Fourier crop to box size (pix): Size of outputs bins after downsampling
Lowpass resolution (A): Resolution of corner frequency in Angstroms
Recenter using aligned shifts: Activate this parameter to recenter the particles based on their shifts.
Particles will only be recentered if this is true, and alignments3D or alignments2D are connected.
Note that alignments2D will be used for recentering only if alignments3D are unavailable.
Job: Volume Alignment Tools may be used to change the geometric center of a particle stack prior to downsampling and recentering, for the purpose of subparticle cropping.
Save results in 16-bit floating point: Saves the output particles in float16 format (CryoSPARC v4.4+).
Common Next Steps
Running 2D Classification, 3D Classification, 3D Variability Analysis or Heterogeneous Refinement on downsampled particles. To refine a structure using the full particle resolution, you can reference the original (non-downsampled) particle data using an input-group override as described in this tutorial.