Job: Blob Picker
Blob-based picking.
Description
Automatically picks particles by searching for Gaussian signals.
Input
Aligned/motion-corrected micrographs
Common Parameters
The
Minimum and Maximum particle diameters (A)
to pick are required to use the blob picker. It is recommended to underestimate the minimum diameter and overestimate the maximum diameter.
Output
Particles
Notes and Limitations
Blob picking is a fast picking method but is generally the least accurate. This accuracy issue stems from being overambitious in micrographs featuring Gaussian shaped particles, and performing poorly on micrographs that lack Gaussian-like particles. The former is caused by the blob picker picking all Gaussians, regardless of whether they are particles or not. However, the incorrect particles can be filtered using a combination of the 2D Classification and Select 2D jobs.
Common Next Steps
2D Classification and Select 2D to generate templates for Template picking
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