Job: Gctf (Wrapper)


Estimate CTF parameters of micrographs using Gctf 1.06. CryoSPARC v2 provides a wrapper to Gctf: Jack (Kai) Zhang. Zhang, K. (2016). Gctf : Real-time CTF determination and correction. Journal of Structural Biology, 193(1), 1-12.


  • Aligned/motion-corrected micrographs

Common Parameters

  • Only process this many micrographs: Set a value if you wish to estimate CTF for only a subset of all micrographs, e.g., if you want to build a template for automatic particle picking.


  • Micrographs with CTF information

Common Next Steps

  • Particle picking