Job: MotionCor2 (Wrapper)

Description
An algorithm to correct anisotropic image motion at the single pixel level across the whole frame, suitable for both single particle and tomographic images. Iterative, patch-based motion detection is combined with spatial and temporal constraints and dose weighting. Please ensure you review the MotionCor2 License Terms below.
Input
Raw movies (with microscope parameters & gain reference files imported earlier)
Common Parameters
Path to MotionCor2 executable: The absolute path to the MotionCor2 binary. Note: Run cryosparcm cli 'set_project_param_default(proect_uid, 'exec_param', path)' (where project_uid is your project number (e.g 'P12') and path is the absolute path to the MotionCor2 binary) to keep this parameter consistent across this project.
Output
Global and local (patch-based) motion corrected dose weighted micrographs
Global and local (patch-based) motion corrected non-dose weighted micrographs (for CTF Estimation)
Global and local (patch-based) motion trajectories
MotionCor2 License Terms
1
CryoSPARC does not distribute MotionCor2 binaries. Please ensure you have your own copy of MotionCor2 installed under the terms of the MotionCor2 Non-Commercial Software License Agreement available at: https://docs.google.com/forms/d/e/1FAIpQLSfAQm5MA81qTx90W9JL6ClzSrM77tytsvyyHh1ZZWrFByhmfQ/formResponse.
2
For-profit users must contact David Agard for licensing information prior to download. Structura Biotechnology Inc. makes no warranty regarding MotionCor2.
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Common Next Steps
CTF Estimation (ensure non-dose weighted micrographs are used)

Job: Local motion correction (Multi-GPU)

CTF Estimation

Last modified 5mo ago

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Contents

MotionCor2 License Terms

Common Next Steps