To produce optimal results in publications and for model-building, it is often necessary to re-sharpen and adjust the B-factor of a volume after completing a refinement. This is done by the Sharpening Tools job.
A map volume
A sharpened map
B-Factor to apply: A negative value. A general rule of thumb is to start with the B-Factor value presented in the Guinier Plot of a refinement job (e.g. -76.7)
Common Next Steps
Downloading and inspecting the sharpened map in a software such as UCSF Chimera
Download options for the "volume" output group in Sharpening Tools