Nor press the "+" in the header. A modal window appears to enter new project details.
Non your keyboard. Alternatively, select
New Workspacefrom the Project Details panel on the right side of the screen. Set a Title (may be changed later) and optionally a description.
cryosparcm downloadtestwhile in this directory. This downloads a subset of the T20S dataset.
tar -xf empiar_10025_subset.tarto decompress the downloaded data.
Bon your keyboard, or press the "Building" badge on the job card.
Movies data path: Press the file browse icon and select the movie files (.mrc or .tif format). To select multiple files, use a wildcard, e.g.,
*.mrc. This selects all files that match the wildcard expression. The file browser displays the list of selected files along with the number of matches at the bottom. For this tutorial dataset, navigate to the directory where the test data was downloaded. Use the wildcard expression
*.tifto select all TIFF format movies in the folder. There should be 20 imported movies.
Gain reference pathwith the file browser: Select the single
.mrcfile in the folder where the test data was downloaded.
Dicon changes to a green
S. This indicates the parameter is different from its default value.
Queueto start the import. Use the subsequent modal to select a lane/node on which to run the job. The available lanes depend on your installation configuration. Select a lane, then press
×on the top-right.
exposuresoutput from the previously completed patch CTF estimation job. Drag and drop these into the
Micrographplaceholder in the Job Builder. Queue the job.
Box size: We generally recommend selecting a box size that is at least double the diameter of the particle. In this case, enter a box size of 384. The box size controls how much of the micrograph is cropped around each particle location. Larger box sizes capture the most high-resolution signal that is spread out spatially due to the effect of defocus (CTF) in the microscope. However, larger box sizes significantly increase computation expense in further processing.
# of particles: The total number of particles in each class
Resolution: The relative resolution of all particles in the class (Å)
ECA: Effective classes assigned
map_sharp, the final refined volume with automatic B-factor sharpening applied and filtered to the estimated FSC resolution.