Manually Curate Exposuresis an interactive job in cryoSPARC that enables a user to visually inspect and curate a set of micrographs or movies, using either population-level statistics and adjustable thresholds, or individual inspection of diagnostic plots.
Patch Motion Correctionand
Patch CTF estimation, you can use
Manually Curate Exposuresto help you identify and exclude micrographs with sub-optimal characteristics, e.g., broken or thick ice, carbon edges, too much motion, etc.
local motion correction: Once you have picked particles and estimated location motion trajectories, you can use this information in
Manually Curate Exposuresto identify and exclude micrographs associated with those picks whose local motion trajectories deviate from the norm for your dataset. In this case, the job will output a filtered set of micrographs as well as particles.
Manually Curate Exposuresfrom the Job Builder:
Queueto launch the job.
Overview. An explanation of the
Individualview can be found below.
Y-Axisdrop-down menus directly below allow you to select different statistics by which to plot the micrographs. Selecting a different value from the drop-down will automatically refresh the plot. Common plots include:
Done, the job will finish running and a list of outputs will become available. These can be used for further processing as required.
2D Classificationjob, and/or starting an
Ab-Initio Reconstructionusing only these particles, followed by
micrographs_acceptedto continue processing particle picking and local motion correction using only the good data