Manually Curate Exposures
is an interactive job in cryoSPARC that enables a user to visually inspect and curate a set of micrographs or movies, using either population-level statistics and adjustable thresholds, or individual inspection of diagnostic plots.Patch Motion Correction
and Patch CTF estimation
, you can use Manually Curate Exposures
to help you identify and exclude micrographs with sub-optimal characteristics, e.g., broken or thick ice, carbon edges, too much motion, etc.local motion correction
: Once you have picked particles and estimated location motion trajectories, you can use this information in Manually Curate Exposures
to identify and exclude micrographs associated with those picks whose local motion trajectories deviate from the norm for your dataset. In this case, the job will output a filtered set of micrographs as well as particles.Manually Curate Exposures
from the Job Builder:Queue
to launch the job.Overview
. An explanation of the Individual
view can be found below.X-Axis
and Y-Axis
drop-down menus directly below allow you to select different statistics by which to plot the micrographs. Selecting a different value from the drop-down will automatically refresh the plot. Common plots include:Done
to exit and complete the job. micrographs_accepted
output of the job, micrographs in red will be included in the micrographs_rejected
output, but a selection (blue highlight) will not affect the output unless the selection is accepted or rejected.Done
to exit and complete the job.
Note that all micrographs in green will be included in the micrographs_accepted
output of the job, micrographs in red will be included in the micrographs_rejected
output, but a selection (blue highlight) will not affect the output unless the selection is accepted or rejected.Done
, the job will finish running and a list of outputs will become available. These can be used for further processing as required.particles_accepted
through a 2D Classification
job, and/or starting an Ab-Initio Reconstruction
using only these particles, followed by Refinement
micrographs_accepted
to continue processing particle picking and local motion correction using only the good data